Enhanced stability of recombinant keratinocyte growth factor by mutagenesis
نویسندگان
چکیده
منابع مشابه
cloning and expression of human keratinocyte growth factor in escherichia coli for recombinant drug production
results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...
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Here we demonstrate that keratinocyte growth factor (KGF) and FGFR2IIIb signaling can affect development and function of thymic epithelium (TE) and that alphabeta-lineage thymocytes contribute to intrathymic levels of KGF. Thymocyte expression of KGF is developmentally regulated, being undetectable in CD3-4-8- thymocytes and expressed at highest levels by mature CD4 or CD8 thymocytes. Exposure ...
متن کاملReceptor-mediated endocytosis of keratinocyte growth factor.
Keratinocyte growth factor (KGF) is a fibroblast growth factor which acts specifically on epithelial cells, regulating their proliferation and differentiation. KGF elicits its activity through binding to and activation of KGF receptor, a splicing transcript variant of fibroblast growth factor receptor 2 (FGFR2). Here we analyzed the pathway of internalization of KGF and its receptor using sever...
متن کاملExpression of biologically active recombinant keratinocyte growth factor. Structure/function analysis of amino-terminal truncation mutants.
Keratinocyte growth factor (KGF) is a newly identified member of the fibroblast growth factor (FGF) family (FGF-7). KGF is expressed by stromal fibroblasts and acts on epithelial cells in a paracrine mode. To facilitate structure/function studies, we utilized the T7 prokaryotic expression system to synthesize this growth factor. Recombinant KGF (rKGF) was mitogenic with a specific activity arou...
متن کاملEffect of One Time Irradiation of Uvb Non Keratinocyte Growth Factor Gene Expression in Mice
Purpose: Skin is continuously exposed to many hazardous environmental factors such as ultraviolet radiation (UV). Many investigations have been shown to cause skin damages. The aim of present research was to study the effect of a single time of UVB radiation on the expression pattern of keratinocyte growth factor (KGF) gene in mice. Materials and Methods: UVB (30 mJ/cm2 and 50 mJ/cm2) were rad...
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ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 2006
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/gzj013